Components of the GE2270 complex produced by Planobispora rosea ATCC 53773.
نویسندگان
چکیده
40 : 40 : 20, respectively. Factor T was purified by eluting with acetonitrile water 60 : 40. The purified antibiotics precipitated from the elutes after elimination of the acetonitrile under vacuum.GE2270factors were obtained as white powders showing HPLCretention times and molecular weights reported in Table 1. Factor T showed UV spectra with ^MCl 250, 280 (sh), 345 (sh); ^maxN KOH 240 (sh), 290 (sh), 345 (sh); /l^sphate buffer pH 7'38 250 (sh), 310 (sh), 345 (sh); A|£á" 240 (sh), 275 (sh), 290 (sh), 311, 335 (sh). The other factors showed identical
منابع مشابه
A Genomic, Transcriptomic and Proteomic Look at the GE2270 Producer Planobispora rosea, an Uncommon Actinomycete
We report the genome sequence of Planobispora rosea ATCC 53733, a mycelium-forming soil-dweller belonging to one of the lesser studied genera of Actinobacteria and producing the thiopeptide GE2270. The P. rosea genome presents considerable convergence in gene organization and function with other members in the family Streptosporangiaceae, with a significant number (44%) of shared orthologs. Pat...
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GE2270 is a thiopeptide antibiotic generated by extensive posttranslational modifications of a ribosomally generated precursor peptide. Thiopeptides are especially active against Gram-positive bacteria, including methicillin resistant Staphylococcus aureus (MRSA). In this study the GE2270 biosynthetic gene cluster (pbt) from Planobispora rosea ATCC 53733 was successfully expressed in the hetero...
متن کاملAntibiotic GE2270 a: a novel inhibitor of bacterial protein synthesis. II. Structure elucidation.
GE2270 A, produced by Planobispora rosea ATCC 53773, inhibits Gram-positive bacteria and anaerobes by acting on the bacterial protein synthesis. The structure has been determined by physico-chemical methods applied to the intact molecule and to the main hydrolysis products. Characterization by UV, IR, NMR (double quantum filter COSY), acid-base ionization, elemental analysis and FAB-MS indicate...
متن کاملAntibiotic GE2270 a: a novel inhibitor of bacterial protein synthesis. I. Isolation and characterization.
A novel antibiotic, GE2270 A, was isolated from the fermentation broth of a strain of Planobispora rosea. The product was found to inhibit bacterial protein synthesis. Structural characteristics showed similarities between GE2270 A and thiazolyl peptides such as micrococcin which is known to inhibit protein synthesis by acting directly on the ribosome. Despite this similarity GE2270 A showed fu...
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BACKGROUND A bacterial artificial chromosomal library of Planobispora rosea, a genetically intractable actinomycete strain, was constructed using Escherichia coli-Streptomyces artificial chromosome (ESAC) and screened for the presence of genes known to be involved in the biosynthesis of antibiotics. RESULTS One clone with a 40 kb insert showed antimicrobial activity against Gram positive bact...
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ورودعنوان ژورنال:
- The Journal of antibiotics
دوره 48 9 شماره
صفحات -
تاریخ انتشار 1995